Degradation of morpholine and thiomorpholine by an environmental Mycobacterium involves a cytochrome P450. Direct evidence of intermediates by in situ 1H NMR - Université de Lorraine Access content directly
Journal Articles Journal of Molecular Catalysis B: Enzymatic Year : 1998

Degradation of morpholine and thiomorpholine by an environmental Mycobacterium involves a cytochrome P450. Direct evidence of intermediates by in situ 1H NMR

Abstract

A strain of Mycobacterium sp. RP1 was isolated from a contaminated activated sludge. It was capable of utilizing morpholine, a waste of chemical industry, as sole source of carbon, nitrogen and energy. The kinetic of biodegradation of morpholine was followed directly on the incubation medium using in situ 1 H NMR. This technic allowed to identify two Ž .intermediates of the degradative pathway: glycolate and 2- 2-aminoethoxy acetate. The inhibitory effects of metyrapone on the degradative abilities of the strain RP1 indicated the involvement of a cytochrome P450. This observation was confirmed by spectrophotometric analysis and 1 H NMR. Metyrapol, a known metabolite of metyrapone, was also found to be an inhibitor. The same study of degradation of thiomorpholine showed the formation of sulfoxide, which confirmed the presence of a cytochrome P450.

Dates and versions

hal-03163381 , version 1 (09-03-2021)

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P. Besse, B. Combourieu, Pascal Poupin, M. Sancelme, N. Truffaut, et al.. Degradation of morpholine and thiomorpholine by an environmental Mycobacterium involves a cytochrome P450. Direct evidence of intermediates by in situ 1H NMR. Journal of Molecular Catalysis B: Enzymatic, 1998, 5 (1-4), pp.403-409. ⟨10.1016/S1381-1177(98)00019-8⟩. ⟨hal-03163381⟩
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