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Master thesis

Développement de quatre systèmes originaux pour la détection moléculaire des virus des encéphalites équines « exotiques » (encéphalite japonaise, encéphalites équines du Venezuela, de l’Est et de l’Ouest)

Abstract : Arboviruses are mainly tropical diseases caused by a heterogeneous set of arboviruses. Most arbovirus infections are asymptomatic and can circulate "silently", increasing the risk of an epidemic. To prevent such outbreaks, a rapid, cost-effective, simple test that can be deployed directly in the field is of great importance. This study aimed to develop an isothermal reverse transcription loop-mediated amplification (RT-Loop-Mediated Amplification, RT-LAMP) method for the detection of arboviruses responsible for exotic equine encephalitis: Japanese encephalitis virus, Western, Eastern and Venezuelan equine encephalitis viruses. The sensitivity and specificity of the tested LAMP were determined. The slight differences between the sensitivity and specificity of RT-LAMP in comparison with Real-Time RT-PCR demonstrated that RT-LAMP could be used as a reliable and cost-effective method for the diagnosis of arboviruses. In addition, due to its isothermal nature, no expensive equipment is required, thus reducing the cost of each reaction. Therefore, this test is ideal for use in resource-limited settings such as environmental laboratories and clinics in developing countries.
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Master thesis
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https://hal.univ-lorraine.fr/hal-03359769
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Submitted on : Thursday, September 30, 2021 - 2:41:58 PM
Last modification on : Saturday, April 30, 2022 - 4:02:56 PM

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  • HAL Id : hal-03359769, version 1

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Degrâce Batantou. Développement de quatre systèmes originaux pour la détection moléculaire des virus des encéphalites équines « exotiques » (encéphalite japonaise, encéphalites équines du Venezuela, de l’Est et de l’Ouest). Microbiologie et Parasitologie. 2020. ⟨hal-03359769⟩

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