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Master thesis

Etude du parasite Haplosporidium costale chez l’huître Crassostrea gigas

Abstract : This internship focuses on the study of the behavior of the parasite Haplosporidium costale in the oyster Crassostrea gigas. Indeed, this parasite causes 25 to 50% of mortality in Crassostrea virginica in the United States. In France, it was found for the first time in C. gigas in march 2019 by IFREMER following the observation of mortality on these oysters. In order to study its behavior, as a first step, the development of a real-time PCR and an In Situ Hybridization (ISH) method was carried out. Then using these tools, the study of its prevalence and parasitic cycle in C. gigas was sought. With these methods, the presence of parasitic DNA in adult oysters dating from 2005 could be highlighted as well as its presence in spat. A major difference in the presence of parasitic DNA was found between spat from hatchery (35% DNA from H. costal) and natural uptake (0%). ISH allowed the observation of a structure resembling H. costale in a sample from december 2019. To confirm the discovered information, a study over one year with a larger sample would have to be done.
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Master thesis
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Submitted on : Thursday, September 30, 2021 - 3:34:08 PM
Last modification on : Saturday, April 30, 2022 - 4:02:56 PM

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  • HAL Id : hal-03360019, version 1



Morgane Genet. Etude du parasite Haplosporidium costale chez l’huître Crassostrea gigas. Microbiologie et Parasitologie. 2020. ⟨hal-03360019⟩



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