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Molecularly imprinted polymer as a synthetic receptor mimic for capacitive impedimetric selective recognition of Escherichia coli K-12

Abstract : We fabricated an electrochemical molecularly imprinted polymer (MIP) chemosensor for rapid identification and quantification of E. coli strain using 2-aminophenyl boronic acid as the functional monomer. This strain is a modified Gram-negative strain of Escherichia coli bacterium, an ordinary human gut component. The E. coli strongly interacts with a boronic acid because of porous and flexible polymers of the cell wall. The SEM imaging showed that the bacteria template was partially entrapped within the polymeric matrix in a single step. Moreover, this imaging confirmed E. coli K-12 cell template extraction effectiveness. The prepared MIP determined the E. coli K-12 strain up to 2.9 × 104 cells mL−1. The interference study performed in the presence of E. coli variants expressing different surface appendages (type 1 fimbriae or Antigen 43 protein) or Shewanella oneidensis MR1, another Gram-negative bacteria, demonstrated that the bacterial surface composition notably impacts sensing properties of the bacteria imprinted polymer.
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https://hal.univ-lorraine.fr/hal-03394357
Contributor : Sofiane El-Kirat-Chatel Connect in order to contact the contributor
Submitted on : Thursday, October 28, 2021 - 11:52:00 AM
Last modification on : Thursday, October 28, 2021 - 4:08:15 PM

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Nabila Yasmeen, Mathieu Etienne, Piyush Sindhu Sharma, Sofiane El-Kirat-Chatel, Mariela Brites Helú, et al.. Molecularly imprinted polymer as a synthetic receptor mimic for capacitive impedimetric selective recognition of Escherichia coli K-12. Analytica Chimica Acta, Elsevier Masson, 2021, pp.339177. ⟨10.1016/j.aca.2021.339177⟩. ⟨hal-03394357⟩

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