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Conference papers

Reciprocal influence of experimental arthritis on colitis: a model to study extraintestinal manifestations of IBD?

Abstract : Introduction: Inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), are systemic inflammatory diseases affecting 200 000 people in France and overall 3 million people across Europe [1]. Extraintestinal manifestations (EIM) occur frequently in IBD patients [2]. Amongst these associated complications, rheumatologic manifestations are the most frequent. Conversely, gut inflammation is present in approximately twothirds of patients with spondyloarthritis [3]. Understanding the mechanisms underlying the development of such complications in IBD is a prerequisite to improving the management of these patients. Aim of this study was to investigate influence of experimental colitis on arthritis and vice versa. Material and methods: DSS-induced colitis and collagen-induced arthritis (CIA) were concomitantly induced in DBA/1J mice. Briefly, at day 0, arthritis was induced by intradermal injection of type II bovine collagen, and colitis was performed between day 14 and day 21 with DSS dissolved in drinking water. Mice were daily weighed and severity of arthritis was graduated using macroscopic arthritic score and quantified by measuring hindpaws oedema by plethysmography. Colitis severity was evaluated by disease activity index (DAI) and by detection of fecal lipocalin-2 measured by ELISA. At day 41, mice were sacrificed and histological analyzes were realized in intestines and ankles. Results: Clinical parameters showed that induction of colitis during arthritis development delayed disease onset (2 1 day of delay) and reduced its severity (5.75 1.62 in arthritis only group vs. 4.00 1.48 in arthritis + colitis group). Preliminary histological analyses confirmed these observations. Finally, fecal lipocaline-2 was reduced (50%-decrease) in animal suffering from arthritis and colitis when compared to mice with colitis only. Discussion/Conclusion: These preliminary results indicate that concomitant experimental colitis could influence arthritis in mice and vice versa as demonstrated by reduction of disease severity index and fecal lipocalin 2. References: [1] Bourikas LA. and Papadakis KA. Inflamm Bowel Dis. (2009) 15, 1915– 1924. [2] Leclerc-Jacob S. and al. Aliment Pharmacol Ther. (2014) 39, 957–962. [3] Van Praet L. and al. Nat Rev Rheumatol. (2012) 8, 288–295.
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Contributor : Jean-Yves Jouzeau Connect in order to contact the contributor
Submitted on : Tuesday, March 8, 2022 - 4:54:00 PM
Last modification on : Thursday, March 10, 2022 - 3:31:39 AM


  • HAL Id : hal-03602064, version 1


Julie Hablot, Tunay Kökten, Jean-Yves Jouzeau, Harry Sokol, Laurent Peyrin Biroulet, et al.. Reciprocal influence of experimental arthritis on colitis: a model to study extraintestinal manifestations of IBD?. 20ème congrès de la Société Française de Pharmacologie et de Thérapeutique (SFP&T), SFP&T, Apr 2016, NANCY, France. pp.75 (PS-123). ⟨hal-03602064⟩



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