HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Gold nanostructured membranes to concentrate low molecular weight thiols, a proof of concept study

Abstract : Thiols are very important molecules in the biomedical field involved for example in redox homeostasis. Their detection and quantification remain difficult due to their poor stability (oxidation) linked to their strong reactivity towards other thiols (by the formation of S-S bonds) or other interfering molecules in the medium. Cellulose membranes with immobilized gold nanoparticles (AuNP) were developed to capture and quantify thiols in simple and complex matrices. This device was first optimized and characterized in terms of nanostructuration and thiol adsorption. N-Acetylcysteine (NAC) and reduced glutathione (GSH), chosen as model molecules, were filtered through the device demonstrating a maximal adsorption capacity of 270 and 60 nmol respectively. In a second step, the adsorbed species were subjected to ligand exchange using a more reactive thiol, dithiothreitol. The results showed release rates of approximately 90% for NAC and GSH. Finally, the amount of endogenous GSH in rat plasma was determined without any pretreatment. For the first time to our knowledge, a nanostructured device for the capture, selective and sensitive quantification of thiols is proposed. This device is easy to handle and overcomes matrix effects. Moreover, the very large concentration factor induced by this technology will be a valuable asset to decrease the quantification limits of analytical methods.
Document type :
Journal articles
Complete list of metadata

Contributor : Thomas Chaigneau Connect in order to contact the contributor
Submitted on : Wednesday, April 13, 2022 - 2:24:11 PM
Last modification on : Wednesday, April 20, 2022 - 2:08:36 PM



Margaux Berthou, Arnaud Pallotta, Jordan Beurton, Thomas Chaigneau, Athanassia Athanassiou, et al.. Gold nanostructured membranes to concentrate low molecular weight thiols, a proof of concept study. Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2022, 1198, pp.123244. ⟨10.1016/j.jchromb.2022.123244⟩. ⟨hal-03640308⟩



Record views