Dose-response relationships of indicator-PCBs in orally exposed piglets: assessment of linearity and concentrations in individual tissues - Université de Lorraine Access content directly
Poster Communications Year : 2012

Dose-response relationships of indicator-PCBs in orally exposed piglets: assessment of linearity and concentrations in individual tissues

Abstract

Children are known to be a sensitive population which is potentially exposed to polychlorobiphenyls (PCBs) through soil ingestion because of their hand-to-mouth activity. Therefore, it appears essential to characterize oral bioavailability of PCBs and to assess the PCBs dose-response relationships. Young swine model was designed to determine the relative oral bioavailability of contaminants to young children and is a useful tool in health risk assessment. The present study investigated the oral bioavailability of indicator PCB (I-PCBs) in piglets and their dose-response relationships into selected tissues. Eight young swine male, individually housed, were orally exposed one of the four experimental mixtures (2 replicates) during 10 days. The four experimental mixtures were contaminated by graded levels of a corn oil spiked by Aroclor 1254 to achieve 190, 560, 940 and 1 310 ng I-PCB.Kg-1 of Body Weight (BW) per day. I-PCBs concentration in adipose and cerebral tissues, liver and muscle were determined for each congener. I-PCBs were extracted by Accelerated Solvent Extraction, analyzed by GC-MS and Limits of Quantification (LOQs) were estimated. For PCB28 and PCB52 congeners, levels measured in different tissues were below the LOQs. Regression analysis for other congeners demonstrated significant linear relationships between the administrated doses and the levels measured in adipose tissue (R²>0.70), liver (R²>0.77) and muscle (R²>0.77). The highest levels of I-PCBs and regressions coefficients were found in the liver tissue. Cerebral samples exhibited a nonlinear relationship; however this result needs to be confirmed by further experiments. The dose-response relationship in a range of dose of 190 ng to 1310 ng of Aroclor 1254 I-PCB per Kg of BW appeared to be linear in all studied tissues except the cerebral one. This result has to be considered as a first step for further relative bioavailability studies involving complex matrices like food, soil or dust.
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Dates and versions

hal-03945055 , version 1 (18-01-2023)

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Attribution - NonCommercial - NoDerivatives

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  • HAL Id : hal-03945055 , version 1

Cite

Matthieu Delannoy, Thibault Duval, Agnès Fournier, Guido Rychen, Cyril Feidt. Dose-response relationships of indicator-PCBs in orally exposed piglets: assessment of linearity and concentrations in individual tissues. 7th PCB WORKSHOP, May 2012, Arcachon, France. ⟨hal-03945055⟩
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