An impedimetric aptasensor for ultrasensitive detection of Penicillin G based on the use of reduced graphene oxide and gold nanoparticles - Université de Lorraine Access content directly
Journal Articles Microchimica Acta Year : 2019

An impedimetric aptasensor for ultrasensitive detection of Penicillin G based on the use of reduced graphene oxide and gold nanoparticles

Abstract

The authors describe an impedimetric aptasensor for Penicillin G (PEN) which is an important antibiotic. The method is based on the use of a pencil graphite electrode (PGE) modified with reduced graphene oxide (RGO) and gold nanoparticles (GNPs) for ultrasensitive detection of PEN. The morphology of a bare PGE, RGO/PGE, and GNP/RGO/PGE, and the functional groups on graphene oxide (GO) and RGO were studied using scanning electron microscopy and Fourier transform infrared spectroscopy. Electrochemical impedance spectroscopy was used for detection of PEN by measuring the charge transfer resistance (Rct). Also, cyclic voltammetry was recorded at potential range of 0.30 to +0.70 V for PGE treatment. This aptamer-based assay has a wide linear range that extends from 1.0 fM to 10 μM, and a limit of detection as low as 0.8 fM. The method was applied to the determination of PEN in spiked milk from cow, sheep, goat and water buffalo. Recoveries ranged from 92% to 104%. The assay is fast, ultrasensitive, high reproducible, and selective over antibiotics such as streptomycin, tetracycline, and sulfadiazine.
No file

Dates and versions

hal-04011100 , version 1 (02-03-2023)

Identifiers

Cite

Ayat Mohammad-Razdari, Mahdi Ghasemi-Varnamkhasti, Zahra Izadi, Ali Ensafi, Sajad Rostami, et al.. An impedimetric aptasensor for ultrasensitive detection of Penicillin G based on the use of reduced graphene oxide and gold nanoparticles. Microchimica Acta, 2019, 186 (6), pp.372. ⟨10.1007/s00604-019-3510-x⟩. ⟨hal-04011100⟩
24 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More