Gelatin Methacryloyl (GelMA) hydrogel scaffolds : predicting physical properties using an experimental design approach - Université de Lorraine Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2023

Gelatin Methacryloyl (GelMA) hydrogel scaffolds : predicting physical properties using an experimental design approach

Kamil Elkhoury
Loïc Stefan
Michel Linder
Cyril J.F. Kahn

Abstract

There is a growing interest for complex in vitro environments that closely mimic the extracellular matrix and allow cells to grow in microenvironments that are closer to the one in vivo. Protein-based matrices and especially hydrogels can answer this need, thanks to their similarity with the cell microenvironment and their ease of customization. In this study, an experimental design was conducted to study the influence of synthesis parameters on the physical properties of gelatin methacryloyl (GelMA). Temperature, ratio of methacrylic anhydride over gelatin, rate of addition, and stirring speed of the reaction were studied using a Doehlert matrix. Their impact on the following parameters was analyzed: degree of substitution, mass swelling ratio, storage modulus (log(G’)), and compression modulus. This study highlights that the most impactful parameter was the ratio of methacrylic anhydride over gelatin. Although, temperature affected the degree of substitution, and methacrylic anhydride addition flow rate impacted the gel’s physical properties, namely, its storage modulus and compression modulus. Moreover, this experimental design proposed a theoretical model that described the variation of GelMA’s physical characteristics as a function of synthesis conditions.
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hal-04204953 , version 1 (13-11-2023)

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Corentin Peyret, Kamil Elkhoury, Sabine Bouguet-Bonnet, Sophie Poinsignon, Corentin Boulogne, et al.. Gelatin Methacryloyl (GelMA) hydrogel scaffolds : predicting physical properties using an experimental design approach. International Journal of Molecular Sciences, 2023, 24 (17), pp.13359. ⟨10.3390/ijms241713359⟩. ⟨hal-04204953⟩
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