Molecular Insights for Alzheimer’s Disease: An Unexplored Storyline on the Nanoscale Impact of Nascent Aβ 1–42 toward the Lipid Membrane - Université de Lorraine Accéder directement au contenu
Article Dans Une Revue ACS Applied Materials & Interfaces Année : 2023

Molecular Insights for Alzheimer’s Disease: An Unexplored Storyline on the Nanoscale Impact of Nascent Aβ 1–42 toward the Lipid Membrane

Mounir Tarek

Résumé

Deciphering the mechanism of Alzheimer’s disease is a key element for designing an efficient therapeutic strategy. Molecular dynamics (MD) calculations, atomic force microscopy and infrared spectroscopy were combined to investigate β-amyloid (Aβ1-42) peptide interactions with supported lipid bilayers (SLBs). The MD simulations showed that nascent Aβ1-42 monomers remain anchored within a model phospholipid bilayer’s hydrophobic core, which suggests their stability in their native environment. We tested this prediction experimentally, by studying the behavior of Aβ1-42 monomers and oligomers when interacting with SLBs. When Aβ1 42 monomers and oligomers were self-assembled with lipid bilayer and deposited as SLB, they remain within the bilayers. Their presence in the bilayers induces destabilization of the model membranes. No specific interactions between Aβ1 42 and the SLBs were detected when SLBs free of Aβ1-42 were exposed to Aβ1-42. This study suggests that Aβ can remain in the membrane after cleavage by the γ-secretase, and cause severe damage in the membrane.
Fichier principal
Vignette du fichier
Siniscalco_et_al_ACS_AMI_2023_forHAL.pdf (933.82 Ko) Télécharger le fichier

Dates et versions

hal-04245557 , version 1 (17-10-2023)

Identifiants

Citer

David Siniscalco, Grégory Francius, Mounir Tarek, Semiha Kevser Bali, Olivier Laprévote, et al.. Molecular Insights for Alzheimer’s Disease: An Unexplored Storyline on the Nanoscale Impact of Nascent Aβ 1–42 toward the Lipid Membrane. ACS Applied Materials & Interfaces, 2023, 15 (14), pp.17507-17517. ⟨10.1021/acsami.2c22196⟩. ⟨hal-04245557⟩
41 Consultations
47 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More