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Mise au point de nanoparticules polycationiques pour le transfert de gènes

Abstract : The aim of this study is to develop non-viral delivery systems for DNA. Nanoparticles were prepared by using nanoprecipitation and double emulsion methods. The nanoparticles were characterized physicochemical. Cytotoxicity and transfection efficiency of nanoparticles were performed in different cell lines. Cytotoxicity tests based on mitochondrial activity revealed that all type of nanoparticles had limited cytotoxicity, but depending on both the cell type and the nanoparticles concentration. In order to increase transfection efficiency, photochemical internalization was used to improve endosomal release of nanoparticles. All the results revealed the difficulty to elaborate an efficient DNA vector presenting a low cytotoxicity
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  • HAL Id : tel-01746362, version 1


Myriem Gargouri. Mise au point de nanoparticules polycationiques pour le transfert de gènes. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2010. Français. ⟨NNT : 2010NAN10061⟩. ⟨tel-01746362⟩



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