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Biodégradation des Hydrocarbures Aromatiques Polycycliques, sélection et dynamique des populations bactériennes dans la rhizosphère en fonction de la distance aux racines

Abstract : The biodegradation of Polycyclic Aromatic Hydrocarbons (PAH) is mainly performed by microorganisms that can use these compounds as sole source of carbon and energy. Such capacity has been amply studied to use and optimise microbial activity for remediation of contaminated soils The use of plants has been suggested to increase and accelerate biodegradation rate by improving microbial activity. However, biodegradation mechanisms still remain poorly described as the interactions between plant, pollutant and rhizosphere microflora are often complex. A simplified compartmented device was developed to study rhizospheric processes, especially biodegradation of PAH, as a function of distance to roots, where root exudates and/or PAH were the only carbon sources for microbial growth. The development and use of biomolecular techniques (nucleic acid isolation, PCR, RT-PCR, TGGE, hybridization with a degradation gene specific probe) permitted to follow the structure of bacterial communitities. Gradients of phenanthrene biodegradation were observed as a function of distance to roots, in parallel to spatial and temporal variations in bacterial community structure. These bacterial communities, as well as PAH biodegradation rate, also depended on the aromaticity of PAH and were modified by the symbiosis between plant and an arbuscular mycorrhizal fungus.
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http://docnum.univ-lorraine.fr/prive/SCD_T_2004_0011_CORGIE.pdf
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https://hal.univ-lorraine.fr/tel-01746540
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Submitted on : Thursday, March 29, 2018 - 10:39:57 AM
Last modification on : Wednesday, March 24, 2021 - 10:36:02 AM

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Stéphane Corgie. Biodégradation des Hydrocarbures Aromatiques Polycycliques, sélection et dynamique des populations bactériennes dans la rhizosphère en fonction de la distance aux racines. Sciences de la Terre. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN10011⟩. ⟨tel-01746540⟩

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