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Theses

Cartographie génétique et application à l'étude de l'organisation génomique de la différenciation chez l'épicéa commun (Picea abies (L.) Karst.)

Abstract : This thesis is composed two distinct sections. In the first one, two parental linkage maps were constructed based on AFLP, SSR and ESTP markers, using a full-sib family. Linkage groups homology between parental maps, established from 66 common markers, allowed us to integrate both parental maps. The resulting consensus map included 755 markers (661 AFLPs, 74 SSRs, 18 ESTPs, the 5S rDNA and the early cone formation) distributed on 12 linkage groups reflecting the haploid number of chromosomes of Norway spruce. This consensus map which was the first saturated map published in this species was aligned to other published maps of Picea abies and other coniferous species. This comparative mapping showed good conservation of synteny in the different Picea abies maps, but only partial linkage group homology based on a few ESTP markers with Pinus species. In the second section of the thesis, the genomic organisation of geographic differentiation within the natural area of the species was investigated based on AFLP, SSR and ESTP markers previously positioned on the consensus map. Results showed that a small number of markers with significant GST (Nei's genetic differentiation coefficient) values were involved. These differentiated loci were scattered over all linkage groups. Some of them seemed to be linked. Discussion was focused on the glacial and postglacial history of Picea abies in Europe and the evolutionary forces that may affect the genome organisation.
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Theses
File URL :
http://docnum.univ-lorraine.fr/prive/SCD_T_2004_0033_ACHERE.pdf
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https://hal.univ-lorraine.fr/tel-01746541
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Submitted on : Thursday, March 29, 2018 - 10:39:58 AM
Last modification on : Wednesday, September 8, 2021 - 4:02:07 PM

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  • HAL Id : tel-01746541, version 1

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Virginie Acheré. Cartographie génétique et application à l'étude de l'organisation génomique de la différenciation chez l'épicéa commun (Picea abies (L.) Karst.). Sylviculture, foresterie. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN10033⟩. ⟨tel-01746541⟩

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