Skip to Main content Skip to Navigation
Theses

Il genoma delle Tuberaceae : analisi funzionale di sequenze espresse durante la morfogenesi di Tuber borchii Vittad.

Abstract : The ripening of a truffle fruitbody leads to drastic changes in its size, morphology, cellular organization and aroma production. To explore the gene expression in Tuber borchii during fruitbody development, cDNA libraries from fruitbodies at two stages of maturation were constructed, 3000 cDNA clones were isolated, 2043 were used for constructing cDNA arrays and 641 ESTs of high quality were produced. The results demonstrate that 1) the molecular machinery which allows the truffle ripening mostly consists of orphan genes; 2) truffles have an intense metabolism where pathways belonging to the primary and secondary metabolism are active; 3) some stress-related genes can be identified as markers for fruitbody formation. Furthermore the expression pattern of a cDNA sequence, isolated from ripe fruitbody, showing a good similarity to a Neurospora crassa blue-light inducible gene, was studied using Real-Time RT-PCR assay. The data obtained suggest a light-dependent response in T. borchii.
Document type :
Theses
File URL :
http://docnum.univ-lorraine.fr/prive/SCD_T_2004_0196_GABELLA.pdf
Complete list of metadatas

https://hal.univ-lorraine.fr/tel-01746728
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 10:43:34 AM
Last modification on : Friday, February 5, 2021 - 3:43:58 AM

Identifiers

  • HAL Id : tel-01746728, version 1

Collections

Citation

Silvia Gabella. Il genoma delle Tuberaceae : analisi funzionale di sequenze espresse durante la morfogenesi di Tuber borchii Vittad.. Silviculture, forestry. Université Henri Poincaré - Nancy 1, 2004. Italian. ⟨NNT : 2004NAN10196⟩. ⟨tel-01746728⟩

Share

Metrics

Record views

30