Skip to Main content Skip to Navigation
Theses

Démonstration "in vitro" d'un rôle de l'Androgen-Binding Protein sertolienne (ABP) sur la spermiogenèse

Abstract : In the aim to study the ABP effects on spermiogenesis, we have performed a coculture model involving rat ABP producing or non producing Sertoli cells and rat spermatids. We have shown, by quantitative RT-PCR, an ABP effect on Tnp1 gene expression (involved in nuclear compaction),however, no direct effect of ABP on Spag4 gene expression (involved in sperm tail formation) was observed. Secondly, we have screened spermiogenesis gene expression by DDRT-PCR in the presence of ABP. Among the differential cDNA bands, we have identified a cDNA fragment identical to the mRNA of the mouse calcyclin gene (99 %). By real-time RT-PCR, we have demonstrated a negative effect of ABP and spermatids on the Sertoli cell expression of this gene. Our results show for the first time an ABP effect by itself on spermiogenesis in vitro.This effect is modulated by steroid addition.
Document type :
Theses
File URL :
http://docnum.univ-lorraine.fr/prive/SCD_T_2002_0225_DELLA_MARIA.pdf
Complete list of metadata

https://hal.univ-lorraine.fr/tel-01746738
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 10:43:50 AM
Last modification on : Thursday, October 15, 2020 - 11:01:28 AM

Identifiers

  • HAL Id : tel-01746738, version 1

Collections

Citation

Julie Della-Maria. Démonstration "in vitro" d'un rôle de l'Androgen-Binding Protein sertolienne (ABP) sur la spermiogenèse. Biologie moléculaire. Université Henri Poincaré - Nancy 1, 2002. Français. ⟨NNT : 2002NAN10225⟩. ⟨tel-01746738⟩

Share

Metrics

Record views

16