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Etude de l'épimérisation de sels biliaires 7[alpha]-hydroxylés par Clostridium absonum ATCC 27 555

Abstract : Clostridium absonum ATCC 27555 synthesize three inducible hydroxysteroid dehydrogenases which ensure epimerization of 7[alpha]-hydroxylated bile salts. In cultures in fermentor in complex medium, induction of these enzymes by chenodeoxycholate was maximum when this substrate was added during growth, in condition of a limiting effect of glucose and pH on the production of biomass and organic acids. In continuous cultures in chemically defined medium, in the presence of chenodeoxycholate the carbon flows were reoriented favoring the production of acetate and energy, at the expense of the reoxydation of nucleotidic cofactors, so as to ensure epimerization of this acid. Some properties of the purified hydroxysteroid dehydrogenases was studied. These results support the hypothesis that epimerization of chenodeoxycholate may represent a detoxification process.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2003_0158_WARCHOL.pdf
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https://hal.univ-lorraine.fr/tel-01746824
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Submitted on : Thursday, March 29, 2018 - 10:45:33 AM
Last modification on : Wednesday, September 16, 2020 - 2:37:13 PM

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Magda Warchol. Etude de l'épimérisation de sels biliaires 7[alpha]-hydroxylés par Clostridium absonum ATCC 27 555. Biochimie, Biologie Moléculaire. Université Henri Poincaré - Nancy 1, 2003. Français. ⟨NNT : 2003NAN10158⟩. ⟨tel-01746824⟩

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