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Etude des phénomènes de mécanoactivation et de mécanotransduction des chondrocytes

Abstract : The cartilage is an hydrated connective tissue in joints that withstands and distributes mechanical forces. The chondrocytes utilize mechanical signals to regulate their metabolic activity through complex biological and biophysical interactions with the extracellular matrix. The objective of this work was to study the influence of shear stress on cells behavior cultured in monolayer. Then, cells seeded onto 3D biosystems (alginate and alginate with hyaluronate or hyaluronate derivative) were submitted to a mechanical stress by creating collision between the biosystems in order to simulate the traumatisms resulting from accidental shocks. The metabolic activities, the expression of type I and II collagen and CD44 receptors and integrins [alpha]4/[beta]1 and [alpha]5/[beta]1 were the main studied parameters. Lastly, the importance of mechanical stress on cell redifferentiation process was investigated. Our results show that mechanical stress increased the chodrocyte metabolic activity compared with static cultures. As mechanical stimulus caused variations of adhesion molecules, it seems that these receptors can induce a variety of stimulatory signais to regulate chondrocyte metabolic activities in cartilage microenvironment. Moreover, no dedifferentiation was observed under the mechanical stress. On the contrary, it seems that the redifferentiation process was faster under the mechanical stress. Finally, during a mechanical stimulation, the shock wave might be attenuated in matrix with hyaluronate derivative resulting in a significant decrease of synthesis activities.
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Theses
File URL :
http://docnum.univ-lorraine.fr/prive/SCD_T_2002_0297_GIGANT_HUSELSTEIN.pdf
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https://hal.univ-lorraine.fr/tel-01747030
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 10:49:47 AM
Last modification on : Wednesday, October 21, 2020 - 2:59:49 PM

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  • HAL Id : tel-01747030, version 1

Citation

Céline Gigant-Huselstein. Etude des phénomènes de mécanoactivation et de mécanotransduction des chondrocytes. Biotechnologies. Université Henri Poincaré - Nancy 1, 2002. Français. ⟨NNT : 2002NAN10297⟩. ⟨tel-01747030⟩

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