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Theses

Etudes in vitro et in vivo des effets potentiels de trois solutions d'hémoglobine modifiée chimiquement sur les propriétés biologiques des cellules sanguines et endothéliales

Abstract : Several hemoglobin-based solutions are currently being evaluated as possible blood substitutes in transfusion medicine. The solutions have their own physico-chemical properties and are thus likely to induce specific changes in the cardiovascular balance. This work aims to examine the consequences of the injection of three hemoglobin-based oxygen carriers (HBOCs) structurally different on the biological properties of the vascular area cells in order to assess how many the hemostatic and anti-infectious functions were preserved. We have studied, both in vitro and in vivo, in a comparative way, the effects of a hemoglobin conjugated to dextran-benzene-tetracarboxylate (Hb Dex-BTC), a cross-linked hemoglobin (oeoe-Hb) and a polymerized cross-linked hemoglobin (Hemolink[TM]) on endothelial cells, polymorphonuclear neutrophils (major population of leukocytes in humans) and platelets in a model of 20% of the total blood volume hemodilution. We have demonstrated that, in a three hour period, the tested solutions did not alter the neutrophils behaviour but according to their rheological properties, they may affect the platelet and endothelial physiology.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2002_0304_TOUSSAINT_HACQUARD.pdf
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https://hal.univ-lorraine.fr/tel-01747039
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Submitted on : Thursday, March 29, 2018 - 10:49:55 AM
Last modification on : Friday, September 10, 2021 - 1:04:03 PM

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  • HAL Id : tel-01747039, version 1

Citation

Marie Toussaint-Hacquard. Etudes in vitro et in vivo des effets potentiels de trois solutions d'hémoglobine modifiée chimiquement sur les propriétés biologiques des cellules sanguines et endothéliales. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2002. Français. ⟨NNT : 2002NAN10304⟩. ⟨tel-01747039⟩

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