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Contribution à l'étude taxonomique des armillaires africaines et européennes par l'analyse du polymorphisme de l'ADN ribosomal

Abstract : Polymorphism of ribosomal DNA has been studied in order to characterize the African and European Armillaria species. Size polymorphism of the ITS region coupled with RFLP allowed characterising three African species, as well as the T7 isolate, which very likely belongs to the heterothallic A. heimii species. PCR/RFLP techniques, used with sequencing of the ITS region allowed discriminating the seven European species. In addition, it has been shown that A. cepistipes is closely related to A. gallica and A. borealis to A. ostoyea. A. cepistipes was found to be composed of several types. A. ectypa which is exclusively found in peat bogs was clearly different from the ether species. Such molecular tools, combining PCR/RFLP and DNA sequencing appear as efficient and reliable to discriminante and identify a large number of Armillaria species in field conditions.
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http://docnum.univ-lorraine.fr/prive/SCD_T_1996_0259_CHILLALI.pdf
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https://hal.univ-lorraine.fr/tel-01747250
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Submitted on : Thursday, March 29, 2018 - 10:55:44 AM
Last modification on : Wednesday, February 17, 2021 - 2:07:52 PM

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Mohamed Chillali. Contribution à l'étude taxonomique des armillaires africaines et européennes par l'analyse du polymorphisme de l'ADN ribosomal. Sciences de la Terre. Université Henri Poincaré - Nancy 1, 1996. Français. ⟨NNT : 1996NAN10259⟩. ⟨tel-01747250⟩

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