Intérêt des cellules souches mésenchymateuses en ingénierie tissulaire du cartilage : effets des stimuli mécaniques et biochimiques

Abstract : Cartilage tissue engineering could lead to reconstruct a re-implantable neo-cartilage by using cells such as chondrocytes and mesenchymal stem cells (MSCs) cultured in a scaffold. Different mechanical and biochemical factors play an essential role in this process as well. The aim of this study was to investigate firstly, the influence of monolayer expansion of human chondrocytes and MSCs on the cell phenotype. Secondly, the cellular responses of chondrocytes and MSCs to mechanical stimulations (agitation and intermittent dynamic compression) as well as to biochemical stimulations (TGF-pl and BMP-2) have been examined in an alginatelHA hydrogel. Our results showed that notable dedifferentiation of chondrocytes has occurred from passage 2, while MSCs have still kept their undifferentiated phenotype until passage 5. ln addition, the application of these mechanical strains and growth factors has been demonstrated to improve metabolic activity, cell proliferation, chondrogenic gene expression as well as synthesis of cartilaginous matrix. These effects are closely related to the mechanical parameters (duration, frequency) and the cell type.AlI of these results suggested that the control of culture conditions and the application of mechanical strains as well as growth factors could modulate the differentiation state and the chondrogenesis.
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Yun Wang. Intérêt des cellules souches mésenchymateuses en ingénierie tissulaire du cartilage : effets des stimuli mécaniques et biochimiques. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2007. Français. ⟨NNT : 2007NAN11304⟩. ⟨tel-01747415⟩

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