Etude structurale et fonctionnelle du cluster eps de Streptococcus thermophilus IP6756 : spécificités et hypothèses nouvelles

Abstract : The S. thermophilus IP6756 eps cluster organization is very original. On the one hand in that EPS-producing strain only two ORF compose the regulation module instead of four for other eps cluster of this specie and on the other hand, only two ORF form the biosynthesis-export module. Interestingly, none of those ORF products display glycosyltransferase enzyme homology. Functional study of this cluster shows that it is organized in operon and that its polar interruption does not lead to any modification in EPS yield. All these results indicate that the S. thermophilus IP6756 eps cluster is not involved in EPS production (on quantitative terms) and that EPS production is under control of gene(s) elsewhere in the genome, particularly glycosyltransferases genes. Like other S. thermophilus eps clusters, the genome of the IP6756 strain displays orf14.9, whose function is unknown. This ORF is transcribed. After disruption experiments, no links can be established between this ORF function and EPS production, at least on quantitative term. Nevertheless, orf14.9 integrity seems to be necessary to an optimal growth of S. thermophilus.
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Guillaume Tyvaert. Etude structurale et fonctionnelle du cluster eps de Streptococcus thermophilus IP6756 : spécificités et hypothèses nouvelles. Génétique. Université Henri Poincaré - Nancy 1, 2005. Français. ⟨NNT : 2005NAN10123⟩. ⟨tel-01748161⟩

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