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Etude in situ, par spectroscopie infrarouge en mode ATR, des premières étapes de la formation d'un biofilm de Pseudomonas fluorescens et de sa réponse aux variations de la quantité de carbone organique dissous : application à la détection précoce du changement de la qualité microbiologique d'une eau de distribution

Abstract : Drinking water biostability can evolve during its transport through distribution systems because of several factors. Bacterial biofilms which develop on the inner surface of pipes can detach and can consequently be a major source of contamination. This study evaluates the feasibility to assess, in real time, drinking water biostability by monitoring in situ the evolution of the Attenuated Total Reflectance Fourier Transform InfraRed (ATR-FTIR) fingerprint of a nascent reference biofilm exposed to water under test. Pseudomonas fluorescens CIP 69.13 was chosen as the reference bacteria to form the monolayer reference biofilm. Its IR and Raman fingerprints were studied, in a first step, in planktonic state in accordance with growth phases and degree of hydration. Then, optimal experimental conditions were determined by following in situ and in real time the first steps of bacterial adhesion thanks to an ATR flow cell. Biofilm structure was characterised by Raman microspectroscopy and epifluorescence microscopy. Finally, the reference biofilm response was analysed when biofilm was exposed to different dissolved organic carbon (DOC) quantities in filtered drinking water. This parameter was chosen because its significant role in bacterial regrowth in drinking water distribution system. The last part clearly shows the dynamic response of biofilm growth and the relationship between the spectral profiles changes and DOC variations. Moreover, it illustrates the potential of the developed method to manage drinking water stability.
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https://hal.univ-lorraine.fr/tel-01748269
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Anne Delille. Etude in situ, par spectroscopie infrarouge en mode ATR, des premières étapes de la formation d'un biofilm de Pseudomonas fluorescens et de sa réponse aux variations de la quantité de carbone organique dissous : application à la détection précoce du changement de la qualité microbiologique d'une eau de distribution. Autre. Université Henri Poincaré - Nancy 1, 2007. Français. ⟨NNT : 2007NAN10119⟩. ⟨tel-01748269⟩

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