Développement d'une méthode originale pour l'évaluation de l'activité virucide des antiseptiques - désinfectants : détermination du pouvoir antiseptique de calixarènes sur le coronavirus Humain

Abstract : Efficient antisepsis-disinfection is fundamental, considering the lack in antiviral treatments, the emergence of new viruses and the raising of viral nosocomial infections. Only one European Standard (NF EN 14476 + A1) proposes a frame to evaluate antiseptic antiviral activity in Human medicine and some improvements are still needed. We thus developed and validated, biologically and physico-chemically, a virucidal assay based on an original gel filtration method, using "in-house" G-25 and G-10 Sephadex[trademark] columns, as neutralization method. We evaluated, on the Human coronavirus 229E (HCoV 229E), the antiseptic activity of two reference molecules, chlorhexidine (CHX) and hexamidine (HXM), and of two new potent antiviral drugs: the tetra-para-sulfonatocalix[4]arene (C[4]S) and the 1,3-bis(bithiazolyl)-tetra-para-sulfonato calix[4]arene (C[4]S-BTZ). A 4 log10 reduction in viral titers is required, according to the European Standard.HXM and C[4]S showed almost no activity on the HCoV 229E. Considering the CHX, it showed a quite interesting activity, even if it did not reach the threshold to pretend to an antiseptic activity on the HCoV 229E. Finally, The C[4]S-BTZ showed a comparable activity to the CHX, and even better. Thanks to this original method, we could highlight a new interesting molecule, the C[4]S-BTZ, which showed a close activity to the CHX, but faster, residual and exempt of cytotoxicity, whereas chlorhexidine did.
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Chloé Geller. Développement d'une méthode originale pour l'évaluation de l'activité virucide des antiseptiques - désinfectants : détermination du pouvoir antiseptique de calixarènes sur le coronavirus Humain. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2010. Français. ⟨NNT : 2010NAN10042⟩. ⟨tel-01748553⟩

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