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Étude de l'impact de la nutrition azotée et des conditions de culture sur le contenu en polyphénols chez la tomate

Abstract : During my PhD we studied the effects of nitrate supply on tomato polyphenolics content. Several cultural systems were used (hydroponic, rockwool culture, NFT). We quantified the main tomato phenolic compounds: chlorogenic acid, rutine, kaempferol rutinoside in vegetative parts (leaf, stem, root), together with some caffeic acid derivates and naringenine chalcone in fruits. These compounds were analyses by High Performance Liquid Chromatography. We noticed that leaves were the more responsive compartment of the plant, to the nutrition conditions. We observed a two-fold increase in polyphenolics concentrations when nitrate supply decrease from 15 to 0.05mM. We found, by drawing response curves, that this increase was more important when nitrate supply limited plant growth. Ten to 20 days seem necessary to observe a modification, at the plant level, of polyphenolics content. In tomato fruits, polyphenolics concentrations were modified by the nitrate supply, but we do not observed very significant increases. The effects of other environmental factors, such as climate conditions (light, temperature) were studied. Our results seem to indicate that climate is more important than nitrogen nutrition for the determination of the polyphenolic compounds concentrations
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  • HAL Id : tel-01748724, version 1
  • PRODINRA : 45633

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Camille Bénard. Étude de l'impact de la nutrition azotée et des conditions de culture sur le contenu en polyphénols chez la tomate. Sciences agricoles. Institut National Polytechnique de Lorraine, 2009. Français. ⟨NNT : 2009INPL050N⟩. ⟨tel-01748724⟩

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