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Spectroscopie optique multi-modalités in vivo : instrumentation, extraction et classification diagnostique de tissus sains et hyperplasiques cutanés

Abstract : The incidence of skin cancers is steadily increasing. Their in vivo early diagnosis and characterization is an important issue. An approach noninvasive: the spatially resolved multi-modality spectroscopy has been implemented. The instrumentation developed allows to co-localized measures in multiple AutoFluorescence excitation (AF, 7 peaks between 360 and 430 nm) and Diffuse Reflectance (DR, 390 to 720 nm) spatially resolved at 5 inter-fiber distances (between 271 and 1341 m). The experimental protocol was focused on the early stages of skin cancer UV-induced in a preclinical model. Four reference classes were defined based on the histopathological analysis of the skin samples: Healthy (H), Compensatory Hyperplasia (CH), Atypical Hyperplasia (AH) and Dysplasia (D), leading to 6 combinations of class pairs to be discriminated. After preprocessing of the raw spectra, extraction, selection and reduction of the most discriminative spectroscopic data set were performed. Then, the efficacy of three supervised classification algorithms was compared: k-Nearest Neighbors, Linear Discriminant Analysis and Support Vector Machine. The contribution of the different modalities was also evaluated: single AF excitation alone, Excitation-Emission Matrices AF (EEMs) alone, DR alone, coupling of EEMs and RD, coupling of EEMs and DR with spatial resolution. The final efficiency of our diagnostic method was evaluated in terms of sensitivity (Se) and specificity (Sp). The best results obtained are: Se and Sp 100% for discriminating CH vs others; Sp 100% and Se> 95% for discriminating AH or D vs H; Sp 74% and Se 63% to discriminate AH vs D
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https://hal.univ-lorraine.fr/tel-01748916
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Gilberto Diaz-Ayil. Spectroscopie optique multi-modalités in vivo : instrumentation, extraction et classification diagnostique de tissus sains et hyperplasiques cutanés. Autre. Institut National Polytechnique de Lorraine, 2009. Français. ⟨NNT : 2009INPL085N⟩. ⟨tel-01748916⟩

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