Agrégation de cellules bactériennes par des polymères cationiques (polyéthylèneimine) : influence de la masse moléculaire du polymère et de la présence/absence de surstructures exopolymériques bactériennes sur la déshydratation des boues biologiques

Abstract : After dewatering, a biological sludge still contains an average water content of 70%, thus causing huge environmental problems. To achieve a better understanding of the mechanisms underlying physicochemical interactions inducing water retention in the sludge, the bacterial sludge was modeled by a pure strain producing or not surface appendages, depending on the growth temperature. These bacterial suspensions were flocculated by polyethyleneimine of various molecular weights. Measurements of optical density, electrophoretic mobility, conductivity, pH, absorbance, particle size, as well as AFM and TEM observations were performed. This allowed to characterize aggregates structures and to estimate the influence of molecular weight and concentration of polymer and presence/absence of surface appendages. Measurements of elasticity, spring constant and turgor pressure were carried out by force spectroscopy allowing to characterize nanomechanical properties of aggregates. In a second step, a dewatering of these flocculated suspensions was performed by centrifugation, process used in some wastewater treatment plants. Measurements of elasticity and viscosity were carried out by rheology. Correlations with dryness measurements allowed to conclude that the presence/absence of surface appendages, and also the molecular weight and concentration of the polymer have a significant impact on biological sludge dewatering
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Marie-Eve Krapf. Agrégation de cellules bactériennes par des polymères cationiques (polyéthylèneimine) : influence de la masse moléculaire du polymère et de la présence/absence de surstructures exopolymériques bactériennes sur la déshydratation des boues biologiques. Autre. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0089⟩. ⟨tel-01749281⟩

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