Localisation du ganglion sentinelle au moyen de nanoparticules fluorescentes émettant dans le proche infrarouge : application au cancer du sein

Abstract : Sentinel lymph node (SLN) biopsy is a reliable technique for the diagnosis of metastases in breast cancer. However, the tracers used (blue dye and radiocolloid) are not optimal because they can cause allergic reactions and major costs in waste processing. Our strategy was to use near-infrared emitting nanoparticles for the mapping of SLN: indium-based Quantum Dots (QDs) and cyanine 7 embedded in silica nanoparticles (SiNP). In a murine model of mammary carcinoma, all SLN containing lymphatic metastases could be visualized with fluorescent indium-based QDs. The biodistribution study concluded that the major organs of retention were the injection point and lymph nodes whereas liver and spleen accumulated fewer QDs. The cytotoxicity tests demonstrated a weak in vitro toxicity of indium- compared to cadmium-based QDs. SiNP show several advantages over free fluorophore such as biocompatibility, better retention in the SLN and greatest photophysical properties. SLN could be mapped as soon as 5 minutes after SiNP injection. The in vivo toxicity in mice was followed during 3 months after injection and did not reveal any signs of general or hepatic toxicity. Both fluorescent nanoparticles are thus well adapted for the mapping of the SLN and could be a favourable substitute to the actually tracers
Document type :
Theses
Complete list of metadatas

https://hal.univ-lorraine.fr/tel-01749319
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 12:12:45 PM
Last modification on : Wednesday, April 11, 2018 - 1:29:21 AM
Long-term archiving on : Friday, September 14, 2018 - 8:45:49 PM

File

DDOC_T_2012_0120_HELLE.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01749319, version 1

Citation

Marion Helle. Localisation du ganglion sentinelle au moyen de nanoparticules fluorescentes émettant dans le proche infrarouge : application au cancer du sein. Médecine humaine et pathologie. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0120⟩. ⟨tel-01749319⟩

Share

Metrics

Record views

26

Files downloads

198