Synthesis of stable and non-cadmium containing quantum dots conjugated with folic acid for imaging of cancer cells

Abstract : Semiconductor QDs are tiny light-emitting crystals, and are emerging as a new class of fluorescent labels for medicine and biology. The aim of this work was to develop a new class of non-toxic QDs probes with essential attributes such as water dispersibility, photostability, biocompatibility, high luminescence and possible excitation with low-energy visible light, using simple processing method. Such nanoprobes could be used for bio-imaging of cancer cells. In the performed studies, I focused on ZnS and ZnSe QDs as they are cadmium-free and might be excited biphotonically.The synthesis protocols of ZnS and ZnSe QDs doped with two ions such as Mn or Cu and stabilized by 3-mercaptopropionic acid or 1-thioglycerol were established, followed by NCs characterization (diameter, surface charge, photophysical properties) using analytical techniques such as spectrophotometry UV-vis, fluorimetry, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), dynamic light scattering (DLS), infra-red analysis (FT-IR), thin layer chromatography (TLC) and electron paramagnetic resonance (EPR). The cytotoxicity of synthesized bare and conjugated NPs was evaluated on cancer cell lines using MTT, XTT and ferrous oxidation-xylenol orange assay.Finally, chosen well fluorescent and weakly toxic types of as-prepared and characterized QDs were used for bio-imaging of cancer cells. In these experiments, FA-functionalized NCs were excited biphotonically. The performed experiments showed the potential of QDs as cancer cells fluorescent markers and that they accumulate around the cell nuclei.
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Malgorzata Geszke-Moritz. Synthesis of stable and non-cadmium containing quantum dots conjugated with folic acid for imaging of cancer cells. Human health and pathology. Institut National Polytechnique de Lorraine, 2011. English. ⟨NNT : 2011INPL066N⟩. ⟨tel-01749496⟩

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