Microbiological growth control by nisin, lysozyme and lactic acid combination : application to active packaging

Abstract : Food and packaging are closely related. Many chemical and physical reactions exist between a food, its packages and the environment, which alter the composition, quality and physical properties of the food and/or the package. Thanks to active or antimicrobial packaging, food products can be distributed over a wide geographical area over a long period of time without unacceptable quality loss. Natural antimicrobials such as nisin, lysozyme or lactic acid improve shelf-life, eliminate undesirable pathogens and delay microbial spoilage. The present PhD thesis was focused on the improvement of paper wrapping materials by adding nisin, lactic acid and lysozyme in combination and on the determination of the synergism, which could occur between these antimicrobial agents. Synergy between nisin, lysozyme and lactic acid, which was proved against Listeria monocytogenes CIP 82.110 and was not confirmed against Staphylococcus aureus CIP 4.83 by statistical analysis. The paper, containing a mixture of nisin, lysozyme and lactic acid could ensure a antimicrobial activity against Listeria monocytogenes CIP 82.110 or Staphylococcus aureus CIP 4.93. Nisin diffusion from packaging to simulated food was demonstrated. It was proved that nisin in cellulose was only able to diffuse for 48 h at a speed of 0.03 mg/h. Only 30% of nisin was diffused from paper to gel matrix after 5 days. Extra diffusion analyses on combination of nisin, lysozyme and lactic acid should be performed to confirm the antimicrobial effectiveness of this packaging
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Agnieszka Lucyna Lavigne-Martyn. Microbiological growth control by nisin, lysozyme and lactic acid combination : application to active packaging. Agricultural sciences. Institut National Polytechnique de Lorraine, 2011. English. ⟨NNT : 2011INPL011N⟩. ⟨tel-01749513⟩

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