Etude de l'absorption racinaire du cadmium afin d'améliorer la modélisation de son transfert vers les plantes

Abstract : This thesis aimed to improve the comprehension of cadmium (Cd) absorption by maize and alpine pennycress plants in order to model better its transfer into edible and hyperaccumulating plants. Since the model used is sensitive to the characteristics of root absorption, the first objective was to develop a rigorous method for measuring those parameters. Two protocols were finalized to describe precisely Cd net influx in both root compartments (apoplast and symplast) according to Cd concentration in solution. The results highlighted, for the first time, the existence of a low-affinity transport system (LATS) that works at the same time with the high-affinity transport system (HATS). However, the kinetics parameters measured through these experimentations did not succeed in improving significantly the model: Cd uptake by maize is overestimated by 100%, and Cd uptake by alpine pennycress is under-estimated by 66%. Several factors were investigated in order to understand the reasons of this difference. We showed that the experimental conditions used to measure the kinetics parameters present characteristics that are able to modify their values significantly: the ionic composition of the solution of exposition to Cd, Cd concentration during growth, and the root structure that forms in hydroponics. This thesis suggests to choose aeroponics as controlled culture condition, and to measure the kinetics parameters in an ionic composition that is representative of the close vicinity of the root cell walls and membranes in soil. This composition remains to be investigated.
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Tanegmart Redjala. Etude de l'absorption racinaire du cadmium afin d'améliorer la modélisation de son transfert vers les plantes. Sciences agricoles. Institut National Polytechnique de Lorraine, 2009. Français. ⟨NNT : 2009INPL043N⟩. ⟨tel-01749532⟩

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