Propriétés d'auto-assemblage de phospholipides riches en acides gras polyinsaturés : caractérisation physico-chimique et simulation de bicouches par dynamique moléculaire

Abstract : The literature of recent decades is replete with references regarding the benefits of omega 3 fatty acids such as EPA (C20:5 n-3) and DHA (C22:6 n-3) which play an essential role in preventing many diseases such as neurodegenerative diseases (Alzheimer's type). The major sources of EPA and DHA are those of marine origin. It is within this context that this study chose to deal with phospholipids from salmon heads. The objective was to extract, purify phosphatidylcholine (PC) derived from the mixture of lipids and determine its properties of self-assembly into bilayers. An experimental approach by the physicochemical characterization of this PC was supplemented by a theoretical study of the same compound using the techniques of molecular dynamics simulation that allowed a molecular-scale characterization of lipid bilayers. The characterization resulted in detailed profile of the mixture composition of salmon PC, to draw up the phase diagram of PC-water, to determine the packing and hydration properties of this lipid. The parameters chosen for the study of molecular dynamics have faithfully reproduced the experimental results, thus validating the model and simulation conditions determined in advance. The characterization of structural properties of the PC as a multilayer salmon has deepened the understanding of interaction mechanisms at the molecular level between unsaturated lipids themselves.
Document type :
Theses
Complete list of metadatas

Cited literature [168 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01749542
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 12:19:07 PM
Last modification on : Monday, April 16, 2018 - 10:43:13 AM
Long-term archiving on : Friday, September 14, 2018 - 6:50:45 PM

File

INPL_2011_SAUTOT_P.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01749542, version 1

Collections

Citation

Pascale Sautot. Propriétés d'auto-assemblage de phospholipides riches en acides gras polyinsaturés : caractérisation physico-chimique et simulation de bicouches par dynamique moléculaire. Alimentation et Nutrition. Institut National Polytechnique de Lorraine, 2011. Français. ⟨NNT : 2011INPL032N⟩. ⟨tel-01749542⟩

Share

Metrics

Record views

21

Files downloads

357