Mise au point et validation sur sites contaminés (ETM-HAP) d'un test de biosurveillance en microcosme : croissance et bioaccumulation par gastéropode terrestre Helix Aspersa Aspersa

Abstract : Ecotoxicology is important for the evaluation of ecosystem quality : she studies interactions between chemical and environment and includes several endpoints like establishment of the bioindicator Helix aspersa aspersa. In order to tramspose on field the snail growing test (AFNOR), microcosms previously used were improved to allow the transfer of snails on adverse sites. In the same time, different food combinations were tested to detemine the best compromise between growth and metal bioaccumulation : the combination clover-snail feed was chosen. Several experiments of active biomonitoring were realised on different sites in order to test the efficacy of this new transfer protocol on microcosms: growing rates were significant and accumulation of metals occured Moreover, Helix aspersa aspersa seerns to present interesting capacities for PAH bioindication. In addition to these field experiments, an ecotoxicological study on copper was performed. Results obtained food but don't allow us to conclude on the relationship between metal levels in snail viscera, bacateriological profile evolution and methality. At cellular level, copper intake occurs in degestive cells of the digestive gland then it is transported by some blood cells (phagocytosis and sulphur formation), before excretion by apica vacuoles of nephrocytes.
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Bénédicte Viard-La Rocca. Mise au point et validation sur sites contaminés (ETM-HAP) d'un test de biosurveillance en microcosme : croissance et bioaccumulation par gastéropode terrestre Helix Aspersa Aspersa. Sciences agricoles. Université Paul Verlaine - Metz, 2004. Français. ⟨NNT : 2004METZ035S⟩. ⟨tel-01749557⟩

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