Propriétés interfaciales des virus, concept de particules molles multicouches, corrélation avec les capacités d'adhésion

Abstract : The aim of this work is to characterize the interfacial properties of phages using the soft multilayer particles concept in order to interrelate appropriately their reactivity and their physico-chemical features. It was demonstrated that the RNA inside the capsid do have a major impact on the physico-chemical properties of the virus, in particular its electrophoretic mobility. We further compared electrohydrodynamic features of MS2 phage with those of the corresponding VLPs (virus particles without RNA). In line with theoretical predictions based on the soft particles concept, it is shown that MS2 are more negative than VLPs. The aggregation profiles of both particles significantly differ demonstrating the major influence of the genome on stability of viral suspensions. We then analyzed the influence of the virus purification method on the observed differences in terms of stability and electrohydrodynamics. We also studied the interfacial properties of phages exhibiting similar structures. It appeared that the phages display similar electrostatic charge while their hydrophobicity degree follows the sequence MS2
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Christelle Dika Timbo. Propriétés interfaciales des virus, concept de particules molles multicouches, corrélation avec les capacités d'adhésion. Médecine humaine et pathologie. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0038⟩. ⟨tel-01749570⟩

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