Développement d'un test d'agglutination pour la détection, in situ, de la vitellogénine : biomarqueur de la contamination des écosystèmes aquatiques par oestrogènes mimétiques

Abstract : Estrogen mimics are pollutants present in aquatic environment. These compounds induce abnormalities in the reproductive system of male fishes, which lead to a total or partial male feminization, or to their demasculinization. Finally, these alterations could lead to a disappearance of the total contaminated fish populations. Moreover, these toxics possess the capacity to mimic endogenous estrogens and to induce the abnormal production of vitellogenin (VTG) in male and immature fishes. The purpose of this research was to develop an easy, specific and fast method for the field detection of male fish contamination by estrogen mimics, using VTG as biomarker. The selected method is based on a latex agglutination test (LAT), developed with monoclonal antibodies specific of this biomarker. The development of this VTGLAT, has involved a new purification method of carp VTG in order to produce monoclonal antibodies specifics of this protein. Moreover, it was necessary the optimization of the antibodies adsorption process in terms of particle diameters, pH and antibodies concentration. The optimization of irnrnunoagglutination process was also verified. The VTG-LAT developed possess the capacity to recognize different VTG molecules of several fish species (dace, chub, roach, perch ...) and it is possible by this method to differentiate vitellogenic females from contaminated fishes by estrogen mimics.
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Ilizabete Magalhaes-Antoine. Développement d'un test d'agglutination pour la détection, in situ, de la vitellogénine : biomarqueur de la contamination des écosystèmes aquatiques par oestrogènes mimétiques. Sciences agricoles. Université Paul Verlaine - Metz, 2004. Français. ⟨NNT : 2004METZ025S⟩. ⟨tel-01749578⟩

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