Contribution à la mise au point d'un biomatériau de comblement à base de hyaluronate pour la réparation du cartilage

Abstract : Various hyaluronate derivatives were prepared by covalent fixation of bath alkyl chains (12 carbons) and a difunctional tetraethyleneglycol onto the polysaccharide, via ester functions. The aim of this work was to evaluate the ability of these dually-modified hyaluronate derivatives to form hydrogels, stable in aqueous solution, that can be used as scaffolds for cartilage regeneration. The rheological properties of the derivatives in semi-dilute regime were studied. The dually- modified polysaccharide formed a three-dimensional network, stabilized by intermolecular hydrophobic iftteractions and reinforced by the chemical covalent crosslinks. After an in vitro evaluation of these materials, we demonstrated that, contrary to native hyaluronate, the dually-modified derivatives, embedded in three-dimensional matrices of calcium alginate, were rapidly released from these matrices. ln these conditions, their effect on cell proliferation and proteoglycan syntheses by chondrocytes was consequently difficult to assess. As a new approach to 0btain scaffolds, we investigated the possibility to prepare injectable hydrogels, obtained from derivatives exhibiting a shear-thinning behavior in saline solution and modified with photo sensitive groups, which could form in situ a chemical crosslinking, after the stimulus action.
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Cécile Amargier-Huin. Contribution à la mise au point d'un biomatériau de comblement à base de hyaluronate pour la réparation du cartilage. Alimentation et Nutrition. Institut National Polytechnique de Lorraine, 2005. Français. ⟨NNT : 2005INPL005N⟩. ⟨tel-01749733⟩

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