Élaboration de sondes fluorescentes pour des applications en biologie et imagerie par microscopie de fluorescence

Abstract : Researchs are done with the aim to develop optical methods for biological studies, with the starting point, the fluorescent trac ers chemical synthesis for two biological applications, namely for biopolymer - cells interactions studies and in photodynamic therapy, respectively. In the first part are described the synthesis and the study of bioactive polysaccharides polymers, labeled with fluorescent molecular rotors. The aim was to synthesise and to study the photophysical properties of coumarin derivatives associated with bioactive dextran (carboxymethyldextranbenzylamide (CMDB)) and heparin. The results of the biological tests prove the interest of the study : fluorescent CMBD polymers keep their stimulation effect on endothelial ceUs growth ; moreover, labeling of endothelial and smooth muscle cells with derivatized fluorescent dextran and heparin respectively, has been demonstrated by fluorescence microscopy. The second part is devoted to the development of new photosensitizers for photodynamic therapy (PDT). PDT is a medical treatment against sorne cancers, based on the combined use of a photosensitizer (often porphyrin compound), oxygen and light. Nowadays, compounds used suffer from sorne drawbacks, especially secondary effects and no specificity. This explains the extend of research in this field, to discover more efficient molecules. Our main objective being the targeting, we developed sorne new porphyrins linked with a variable number of glucosamine rings. Different synthetic routes have been explored and now, the synthese protocols are well known. The photophysical properties of compounds have been studied and aggregate formation, even at low concentration has been observed. Biological tests in course, will orientated the further chemical modifications for a good selectivity.
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Pascale Even. Élaboration de sondes fluorescentes pour des applications en biologie et imagerie par microscopie de fluorescence. Sciences agricoles. Institut National Polytechnique de Lorraine, 2001. Français. ⟨NNT : 2001INPL567N⟩. ⟨tel-01749837⟩

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