Synthèse enzymatique d'esters de fructose et d'acide gras : cinétique, purification et caractérisation des propriétés tensioactives

Abstract : This work aims to study kinetics, purification and surfactive properties of sugar esters obtained by enzymatic synthesis. Fructose and various fatty acids (C8 to C18) were used as substrates. These syntheses were carried out in methyl-2 butanol-2, at 60°C and in the presence of an immobilized Candida antarctica lipase as biocatalyst. From the kinetic point of view, the results obtained show that the activity of this enzyme is influenced by substrate concentrations, hydrophobicity and the level of water in the medium. By a judicious control of the synthesis environment, it was possible to convert the quasi totality of the substrates (>90 %) with concentrations bordering the 140 g/1. The monoesters/diesters selectivity was affected by the macro-environment of the system. The purification of the monoesters from the residual substrates was carried out successfully by a liquid-liquid extraction. The purity of the obtained products is about 99 % with losses less than 7%. These extractions were carried out on three sugaresters (C1O, C14 and C18). The characterization of the properties of the sugaresters in a pure state or in mixture with diesters showed that these molecules are as efficient as sucrose esters . They allow to stabilize H/E emulsions and to lower rapeseed oil/water interfacial tensions. In the same way, they have a high foaming capacity.
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Samia Soultani. Synthèse enzymatique d'esters de fructose et d'acide gras : cinétique, purification et caractérisation des propriétés tensioactives. Alimentation et Nutrition. Institut National Polytechnique de Lorraine, 2001. Français. ⟨NNT : 2001INPL592N⟩. ⟨tel-01749881⟩

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