Spectroscopie in vivo de diffusion élastique résolue dans l'espace en combinaison avec la spectroscopie de fluorescence : applications en cancérologie

Abstract : We developed a portable device for "optical biopsy". The device performs in vivo steady state space resolved diffuse reflectance spectroscopy (SRDRS) and light induced fluorescence spectroscopy (LlFS) studies. The originality of the device relies in the use of light emitting diodes (LED) as light source for SRDRS and for fluorescence excitation. We studied the optical properties of healthy and malignant tissues of rat tumor models SM-1, PC-1, Sa-45 and W-256 and mouse tumor model HT29 and Colo 26. The differences in the diffuse reflectance spectra can be explained by differences in scattering properties of those tissues. ln vivo pharmacokinetics of the photosensitiser mTHPBC in rat liver was studied by SRDRS. The precision of the concentration measurements is of the same level as for the chemical extraction studies. However, the use of SRDRS method is more convenient. The developed device can also be applied for LlFS pharmacokinetics studies of photosensitisers such as Foscan (mTHPC).
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Theses
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http://docnum.univ-lorraine.fr/prive/INPL_T_2004_CHALAU_V_N.pdf
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https://hal.univ-lorraine.fr/tel-01750017
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Submitted on : Thursday, March 29, 2018 - 12:36:00 PM
Last modification on : Friday, March 30, 2018 - 1:28:52 AM

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Vadzim Chalau. Spectroscopie in vivo de diffusion élastique résolue dans l'espace en combinaison avec la spectroscopie de fluorescence : applications en cancérologie. Autre. Institut National Polytechnique de Lorraine, 2004. Français. ⟨NNT : 2004INPL068N⟩. ⟨tel-01750017⟩

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