Microscopie de fluorescence en champ proche : fabrication de nano-sondes et visualisation des " rafts " lipidiques dans des couches de Langmuir

Abstract : The aim of this thesis was the study of lipid rafts with scanning probe microscopies, particularly SNOM and AFM. Contrarly to earlier views, lipids do not mix homogeneously in membranes but form domains and micro-domains of different sizes. Glycolipids, such as ganglioside GM1, are receptors for numerous biologically active agents and are likely to be involved in the formation of specialized micro-domains, called «rafts». Characterization of these domains is essential to understand their role in membrane processes. Determination, at the mesoscopic scale, of raft distribution in synthetic and natural membranes has been studied by various techniques and has led to conflicting results: the size of rafts is postulated to be far beyond the resolution of conventional optical microscopy. Langmuir Blodgett films are useful membrane models and are prepared with lipid mixture as a mimic for the natural rafts found in membranes. The characterization of the structure of LB films with SNOM and AFM offers the possibility of obtaining topographic and optical information ranging from nanometer to micrometer resolution. The first project
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Theses
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http://docnum.univ-lorraine.fr/prive/INPL_T_2002_BURGOS_P.pdf
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https://hal.univ-lorraine.fr/tel-01750209
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Submitted on : Thursday, March 29, 2018 - 12:40:35 PM
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Pierre Burgos. Microscopie de fluorescence en champ proche : fabrication de nano-sondes et visualisation des " rafts " lipidiques dans des couches de Langmuir. Sciences agricoles. Institut National Polytechnique de Lorraine, 2002. Français. ⟨NNT : 2002INPL078N⟩. ⟨tel-01750209⟩

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