Spectroscopie d'impédance électrique par biocapteur à micro-électrodes : application à la cytométrie de flux de cellules sanguines

Abstract : This thesis focuses on the implementation and validation of a microfluidic bioimpedance sensor for cytometric measures in the frequency range ( 100kHz - 10MHz ) of biological cells ( blood cells) combined with a microfluidic device. The first chapter introduces the electrical and dielectric properties of living tissues and summarizes the state of the art. The effects of each element of the cells on the overall measured impedance are described, as well as the associated models. A state of the art, on the bioimpedance macroscopic measurements unit cell is outlined in the second chapter. Measurements by flow cytometry and the possible use of surface acoustic wave (SAW) devices as actuators are also studied. The third chapter deals with analytical modeling and simulation by the finite element method of unit cells by microelectrodes of different geometries. 3D simulations were done showing the best configuration for the electrodes design. The results of this section were used to determine the best geometry, their sensibilities, and their answers. The sensor design is described in the fourth chapter. Technological constraints related to its micro- fabrication techniques feasibility and biocompatibility of materials are developed. Flows validation tests were done and are described. The fifth and final chapter focuses on the measurement of cells and particles. In a first step, calibration tests were carried out to determine the form factor of the electrodes and the parasitic impedances. Measurements on cells and particles were used to validate the results obtained in simulation, as well as discrimination based particles tested their dimensions
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Julien Claudel. Spectroscopie d'impédance électrique par biocapteur à micro-électrodes : application à la cytométrie de flux de cellules sanguines. Autre. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0169⟩. ⟨tel-01750347⟩

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