Lipase Mucor miehei immobilisée dans des matériaux poreux silicatés : bioréacteurs pour la synthèse d'esters méthyliques à partir d'huiles végétales

Abstract : This work was to prepare catalysts by immobilizing Mucor miehei lipase into silicate porous materials for the biodiesel production from vegetable oils. To develop this study, several systems based on surfactants were used to synthesize silicate materials with controlled porosity by taking protocols described in the literature or developed in the laboratory. The common point of these materials, whether mesoporous or meso-macroporous, is a hexagonal organization of their mesopores. Meso-macroporous materials were prepared from oil-in- water emulsions (O/W) and from double emulsions (W/O/W) or from solid lipid nanoparticles (SLN). These different materials served as a matrix for immobilization of the lipase. Several methods of immobilization were investigated. Firstly, the lipase was fixed into support by physisorption by immersing material in an enzymatic solution. Secondly, the lipase was linked covalently to matrix with urea bonds. In our case, the material had to be functionalized after this synthesis. Finally, the encapsulation method of the enzyme was carried out only with materials prepared form double emulsions and SLN. All bioreactors were tested by performing a transesterification reaction with methanol. The conditions of this methanolysis were optimized with an experimental design
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Jonathan Jacoby. Lipase Mucor miehei immobilisée dans des matériaux poreux silicatés : bioréacteurs pour la synthèse d'esters méthyliques à partir d'huiles végétales. Alimentation et Nutrition. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0217⟩. ⟨tel-01750513⟩

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