Encapsulation de carbobacterium maltaromaticum LMA28, productrice de composés aromatiques, dans la paraffine d'enrobage du Poro, un fromage artisanal mexicain

Abstract : This thesis focuses on the interdisciplinary study (biochemical, physicochemical, microbiological) in order to improve sensory qualities and, more specifically, aromatic profile of Poro cheese in order to extend its commercialization period on the Mexican market. After cheese characterization, several encapsulations techniques were tested into cheese paraffin coating. Moreover, inhibitory activity of C. maltaromaticum LMA28 isolated from a soft cheese against L. monocytogenes was also studied. Sensory evaluation led to a preferences internal mapping. It appeared that C. maltaromaticum LMA28 encapsulation was a better alternative for the 3-methylbutanal release compared with 3-methylbutanal direct encapsulation to improve off-flavor apparition in Poro. Sensory experiments performed on Babybel® (cheese model) showed a difference between the sensory perceptions of cheese coated containing or not C. maltaromaticum LMA28. Micro-encapsulation in calcium alginate is a quick encapsulation technique, simple, inexpensive and easy to implement even for a small-scale productions. Finally, from a microbiological point of view, no trace of fecal contamination was found in the products. The main strains of lactic acid bacteria isolated were cheese: L. plantarum, L. pentosus, L. farciminis, and L. rhamnosus. The effect of four factors (C. maltaromaticum, NaCl concentration, time and pH) on L. monocytogenes growth, inoculated into two culture media (TSB -YE and skim milk). The C. maltaromaticum LMA28 concentration appeared to be the determining factor for L. monocytogenes inhibition in both media tested
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Citlalli Celeste Gonzalez Ariceaga. Encapsulation de carbobacterium maltaromaticum LMA28, productrice de composés aromatiques, dans la paraffine d'enrobage du Poro, un fromage artisanal mexicain. Alimentation et Nutrition. Université de Lorraine, 2014. Français. ⟨NNT : 2014LORR0038⟩. ⟨tel-01750692⟩

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