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Vieillissement des cellules stromales mésenchymateuses de la moelle osseuse : implications en médecine régénérative

Abstract : Today with their properties of differentiation into specific cells types, mesenchymal stromal cells (MSC) can be used in regenerative medicine. Bone marrow (BM) is the better characterized one. The researchers have proven that with increasing passage number in culture the proliferation and differentiation potential of MSC decrease. In parallel many researchers have showed the impact of donor age on MSC properties remains controversial. The aim of our study was to better understand the effect of donor age but also culture passages on the proliferation and differentiation ability of bone marrow mesenchymal stromal cells. The samples were separated into 4 groups depending on the donor age (<20 years; 20-40 years; 40-60 years; > 60 years) and The samples were cultured for 5 passages. The results obtained show that the MSC proliferative capacity obtained from young donors is greater than that of cells from older donors. In addition, the proliferative capacity decreases with increasing passage number in culture. In parallel, the ability of colony-forming unit-fibroblast, measured by the CFU-F assay, decreases slightly depending on the age of the donors but significantly depending on the passage. Finally, the MSC differentiation ability decreases according to the passage of the cells but also depending on the donor age. Our study shows that the properties of bone marrow derived MSC are modified not only during amplification in vitro but also in terms of donor age
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  • HAL Id : tel-01751794, version 1

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Yueying Li. Vieillissement des cellules stromales mésenchymateuses de la moelle osseuse : implications en médecine régénérative. Médecine humaine et pathologie. Université de Lorraine, 2015. Français. ⟨NNT : 2015LORR0099⟩. ⟨tel-01751794⟩

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