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Étude de la dissémination des cellules tumorales liée à l’acte chirurgical dans les carcinomes epidermoïdes des voies aérodigestives supérieures

Abstract : Metastasis is defined as the development of secondary tumor sites related to the ability of tumor cells to detach from primary tumor, to implant in another organ and to proliferate. From the primary site, a micrometastatic dissemination can occur through the release in blood stream or lymph system of isolated tumor cells or of small cell clusters. These micrometastases can proliferate and grow into metastases. The detection of isolated or microclustered tumor cells, the evaluation of the prognosis value, and their metastatic potential encounter difficulties. In this study, we focused mainly on the metastatic process related to surgery in epidermoid cancers of the upper aerodigestive tract (…). Indeed, in this type of cancers, the best method to establish diagnosis is the biopsy assessed by the anatomo-pathological analysis of a sample. The main physical barrier preventing cells from migrating is the membrane of the malignant tissue. During surgery, these barriers are destroyed, facilitating the invasion of the vascular system. Therefore tumor cells can locate in vessels and proliferate at distance from the primitive site, thus forming secondary tumors. Generally, metastases are detected by imaging or serology at a very advanced stage of cancer disease. The aim of this study was to detect isolated or disseminated cells (CTCs) of CEDVADS in blood stream by three different approaches : 1) the study of molecular markers for the diagnosis of node involvement of epidermoid carcinomas of the upper aerodigestive tract using quantitative PCR in real time and OSNA. 2) Screening of tumoral cells disseminated in Redon drains after cervical curettage 3) Detection of circulating tumor cells after surgery for epidermoid carcinomas of stage III and IV VADS. This protocol will allow to validate the detection of CTCs in clinic setting and to develop prospective studies for the diagnosis and prognosis of CTCs of CEVADS
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Romina Mastronicola. Étude de la dissémination des cellules tumorales liée à l’acte chirurgical dans les carcinomes epidermoïdes des voies aérodigestives supérieures. Médecine humaine et pathologie. Université de Lorraine, 2015. Français. ⟨NNT : 2015LORR0186⟩. ⟨tel-01752180⟩

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