Synthèse d'analogues photoactivables du glutathion : pour l'étude du système cellulaire du glutathion

Abstract : The ubiquitous glutathione (GSH or GSSG) is the topic of many researchs. In most cancers, overexpression of glutathione-dependent enzymes is often found. The aim of this work is to design probes which will be used to better understand the mechanism of apoptosis or to inhibit the glutathione-dependent enzymes in order to sensitise cells to anti-cancer agents. To do that, we have for the first time synthetised photoactivatable N-substituted conjugated analogues of GSH. We have also synthetised new photoactivatable analogues of GSSG with photoactivation wavelengths non-destructive for biological systems. Moreover we have reached key intermediates in the synthesis of other photoactivatable analogues of GSH. The preliminary results of inhibition assays as well as of photolabeling assays performed with some of these analogues allowed us to show that some could serve as probe or as inhibitor of the glutathione-dependent enzymes.
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Dan Bernardi. Synthèse d'analogues photoactivables du glutathion : pour l'étude du système cellulaire du glutathion. Autre. Université Paul Verlaine - Metz, 2005. Français. ⟨NNT : 2005METZ015S⟩. ⟨tel-01752474⟩

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