Influence des facteurs biochimiques et mécaniques in vitro sur la prolifération cellulaire et la synthèse matricielle de fibroblastes : application en Ingénierie tissulaire

Abstract : The process of ligament healing is complex, which consists of the proliferation and the cellular migration, as well as the synthesis of the extra cellular matrix. This process is influenced by the biochemical and/or environmental factors. The specific properties of the ligament cell permit their responses to growth factors and to mechanical stress. The aim of our work was to define the conditions of in vitro reconstruction of ligament tissue. We take into account the biochemical, as well as the mechanical parameters in order to study the factors acting on the synthesis of a new tissue. Firstly, we investigated the influence of the growth factors such as PDGF-AB and TGF-b1 on the cell proliferation and the matrix synthesis (collagens types I and III) respectively. It seems that these two growth factors act on the different mode on the temporal and mechanistic aspects. Secondly, we studied the effect of the specific mechanical stress the stretching on the ligament. It was showed that the action of the mechanical stretching was essential for the differentiation of the target cells and their evolution in the ligament cell, by promoting the synthesis of the specific molecules, such as tenascin, and collagens. Although these results remain insufficient for the reconstruction of neotissue, they allowed us to define the protocols to be followed for the projects of tissue engineering
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Shalaw Grancher. Influence des facteurs biochimiques et mécaniques in vitro sur la prolifération cellulaire et la synthèse matricielle de fibroblastes : application en Ingénierie tissulaire. Autre. Institut National Polytechnique de Lorraine, 2006. Français. ⟨NNT : 2006INPL040N⟩. ⟨tel-01752749⟩

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