Irradiations localisées pour des greffages convalents sur supports

Abstract : This work deals with manufacturing functionalized and patterned plane substrates to immobilize a few biological components like carbohydrates, proteins or antibodies. The substrates are commercial glass microscope slides silanized with an amino linker on the two sides. Covalent binding on such substrates has been successfully tested using fluorophores as models with carboxylic acid or aldehydic function to afford respectively amide or imine bond with the amino functions of the support. To localize chemical graftings, polymeric regular and geometrically controlled wells were manufactured from SU-8 photoresist directly on the glass support, applying stereolithography process. Grafting of two fluorophores with carboxylic functions activated by N-hydroxysuccinimide was led simultaneously inside polymeric wells on a same substrate. Localized bindings were characterized by fluorescence spectroscopy and/or microscopy. In order to enable covalent attachment through a stable chemical linkage of aldehydic components, like antibodies after oxidation of their Fc fragment, aminooxy and hydrazide terminated polyoxyethylene spacers were directly synthesized on substrates surface
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Fanny Evenou. Irradiations localisées pour des greffages convalents sur supports. Autre. Institut National Polytechnique de Lorraine, 2006. Français. ⟨NNT : 2006INPL057N⟩. ⟨tel-01752767⟩

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