Structure spécifique et fonctionnelle de la communauté ectomycorhizienne d'une chênaie-charmaie lorraine

Abstract : The species structure of an ectomycorrhizal (ECM) community bas been assessed monthly during 15 months in a hardwood temperate forest in north-eastern France. Seventy five fungal symbionts associated to tree roots have been identified by morphological and molecular approaches. The species richness of the community and the abundance of each morphotype vary depending on the season and the soil horizon. We have developed a microplate method to measure, on individual ECMs the activities of eight enzymes involved in the degradation of organic matter. The functional profiles of 80 % of the community species were defined monthly. Functional profiles vary between species with the season and the soil horizon. Combining abundance and enzymatic activity measurements led to a synthetic view of the mobilization potential capabilities of the ECM community. Winter and spring correspond to the most active periods of mobilization. A study of the daily modifications of the functional profiles of Lactarius quietus during bud break showed a high correlation between some activities and the modification of' tree physiological parameters. ln addition, a method allowing ECM and soil RNA extraction from forest stand was performed. The transcriptome analysis of ECM species is therefore possible in situ.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2006_0179_COURTY.pdf
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Submitted on : Friday, March 30, 2018 - 9:43:36 AM
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Pierre-Emmanuel Courty. Structure spécifique et fonctionnelle de la communauté ectomycorhizienne d'une chênaie-charmaie lorraine. Sciences de la Terre. Université Henri Poincaré - Nancy 1, 2006. Français. ⟨NNT : 2006NAN10179⟩. ⟨tel-01754280⟩

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