Sélection, identification et caractérisation partielle d'antigènes du spermatozoïde du renard (Vulpes vulpes) en vue de leur utilisation dans un vaccin contraceptif

Abstract : The aim of this work was to identify antigenic proteins on fox (Vulpes vulpes) spermatozoa, in order to develop a contraceptive vaccine. First, we obtained antisera directed against sperm surface proteins dy rabbit and fox immunisation, and by fox vasectomy. Using this sera for Western blotting, 7 highly antigenic proteins were selected. They were named P3, P4, P5, P8, P11 and P13. Out of these 7 proteins, 6 were microsequenced dy using Edman chemistry. Then, we obtained the cDNA encoding the 6 antigens by using RACE PCR or RT-PCR. Two PCR products were used as probes for the screening of a fox testis cDNA library realised in our lab. This manuscript describe a strategy which allowed the selection and the identification of sperm antigens. Many studies are necessary in order to determine the full sequence and properties of these antigens. The determination of specific epitopes and of adapted delivery system could allows their use in a contraceptive vaccine.
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Yann Verdier. Sélection, identification et caractérisation partielle d'antigènes du spermatozoïde du renard (Vulpes vulpes) en vue de leur utilisation dans un vaccin contraceptif. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2002. Français. ⟨NNT : 2002NAN10307⟩. ⟨tel-01754360⟩

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