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Évaluation de l’activité biologique de la galectine 3 et d’une de ses formes tronquées dans la physiopathologie de l’arthrose

Abstract : Osteoarthritis (OA) is a chronic rheumatism which drug management is based on antalgic and anti-inflammatory drugs, which requires the need to search for other therapeutic targets. One of these potential targets may be galectin 3, a lectin secreted in the joint during the pathogenesis of the disease. This lectin binds its ligands on the surface of the joint cells and thus, leads to inflammatory reactions and matrix catabolism phenomena, making it an attractive target in the treatment of OA. The 1st aim of our research project was to characterize the biological activity of galectin 3 on chondrocyte metabolism. The 2nd aim was to design and produce several truncated forms of galectin 3. The 3rd aim was to evaluate the presumed galectin 3-inhibiting activity of the truncated forms. This objective is part from the perspective of gene therapy for OA. Our work shows that galectin 3 is a proinflammatory, procatabolic but also an antianabolic factor in chondrocytes. Several recombinant truncated forms of galectin 3 were designed and produced. Preliminary tests of biological activity in vitro show that the chosen truncated lectin partially inhibits the biological activity of galectin-3 on human chondrocytes under a few conditions. This work is the proof of concept of a broader project of which perspective is gene therapy in OA
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http://docnum.univ-lorraine.fr/prive/DDOC_T_2015_0184_YELEHE_OKOUMA.pdf
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https://hal.univ-lorraine.fr/tel-01754471
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Submitted on : Friday, March 30, 2018 - 9:50:25 AM
Last modification on : Monday, March 2, 2020 - 10:25:24 AM

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  • HAL Id : tel-01754471, version 1

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Mélissa Yéléhé-Okouma. Évaluation de l’activité biologique de la galectine 3 et d’une de ses formes tronquées dans la physiopathologie de l’arthrose. Médecine humaine et pathologie. Université de Lorraine, 2015. Français. ⟨NNT : 2015LORR0184⟩. ⟨tel-01754471⟩

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